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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Adducin alpha, phosphorylated (S59)

Anti -Adducin alpha, phosphorylated (S59)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Serum B IP
Alpha adducin is a 80-120kD member of the aldolase class II family and adducin subfamily. Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; and , or and subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser59, Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).
Catalog #A0859-55G
ApplicationsSuitable for use in Western Blot, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000-5000
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E125
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySerum
ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser59 of human alpha Adducin
SpecificityOnly detects alpha Adducin phosphorylated on Serine 59. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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