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Abs to Actin Related Proteins
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Anti -Adducin, alpha (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADD1, ADDA)
Anti -Adducin, alpha (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADD1, ADDA)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH IF |
|
| Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes. Adducin is a heterodimeric protein that consists of related subunits; alpha and beta, or alpha and gamma subunits. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha. | | | Catalog # | A0859-55P | | Applications | Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence:1:100-1:200 | | Western Blot:1:500-1:1,000 | | Immunohistochemistry:1:50-1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic non-phosphopeptide corresponding to human ADD1 around the phosphorylation site of serine 726 (T-P-S P-F-L). | | Specificity | Recognizes human ADD1. Species Cross-Reactivity: mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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