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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Adducin, alpha (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADD1, ADDA)

Anti -Adducin, alpha (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADD1, ADDA)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes. Adducin is a heterodimeric protein that consists of related subunits; alpha and beta, or alpha and gamma subunits. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha.
Catalog #A0859-55P
ApplicationsSuitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence:1:100-1:200
Western Blot:1:500-1:1,000
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic non-phosphopeptide corresponding to human ADD1 around the phosphorylation site of serine 726 (T-P-S P-F-L).
SpecificityRecognizes human ADD1. Species Cross-Reactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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