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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motifs 4)

Anti -ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin
motifs 4)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP
ADAMTS4 is a member of the family of secreted zinc proteases with a multi-domain structure (1-3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha-2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4-6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). The purified recombinant human ADAMTS4 starts at the catalytic domain and ends before the spacer domain. If desired, the aggrecanase activity can be inhibited by 5mM 1,10-phenanthroline and recombinant human TIMP-3.
Catalog #A0859-60B
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml
Immunoprecipitation: 25ug/ml immunoprecipitates ADAMTS4 from conditioned cell culture medium spiked with recombinant human ADAMTS4.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography
ImmunogenRecombinant corresponding to Phe213-Cys685 from human ADAMTS4 expressedin CHO cells (O75173).
SpecificityRecognizes human ADAMTS4. Shows ~10% crossreactivity with recombinant human ADAMTS5 and <1% crossreactivity with recombinant human ADAMTS13, ADAMTS­L1.2 and ADAMTS1 in Western Blot and ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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