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ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4) (Biotin)
ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4)
(Biotin)
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| ADAMTS4, also known as aggrecanase-1, is a secreted member of the ADAM family of proteins. ADAMTS4 is upregulated by TNF-alpha in osteoarthritis and contributes to joint destruction. Its upregulation in some cancers may enhance tumor invasiveness. Human ADAMTS4 consists of a signal peptide (aa1-51), a pro domain (aa52-212) and a mature chain (aa213-837) containing the following domains: catalytic (aa218-425), disintegrin (aa437-519), TSP type-1 (aa520-575), Cys-rich (aa 577 to 685) and spacer (aa686-837). The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine. | | | Catalog # | A0859-60D | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 0.5-1.0ug/ml | | Western Blot: 1-2ug/ml. The detection limit for rhADAMTS4 is approximately 5ng/lane under non-reducing and reducing conditions. | | Immunoprecipitation: 25ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Product Type | Pab | | Isotype | IgG2a | | Host | Goat | | Source | Human | | Concentration | ~50ug/ml | | Form | Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 50ug BSA, 5% trehalose. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base, 150mM sodium chloride) containing 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NS0-derived, recombinant human ADAMTS4 aa213-685. | | Specificity | Recognizes human ADAMTS4. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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