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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4) (Biotin)

Anti -ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin
motif 4) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B N
ADAMTS4, also known as aggrecanase-1, is a secreted member of the ADAM family of proteins. ADAMTS4 is upregulated by TNF-alpha in osteoarthritis and contributes to joint destruction. Its upregulation in some cancers may enhance tumor invasiveness. Human ADAMTS4 consists of a signal peptide (aa1-51), a pro domain (aa52-212) and a mature chain (aa213-837) containing the following domains: catalytic (aa218-425), disintegrin (aa437-519), TSP type-1 (aa520-575), Cys-rich (aa 577 to 685) and spacer (aa686-837). The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine.
Catalog #A0859-60D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.5-1.0ug/ml
Western Blot: 1-2ug/ml. The detection limit for rhADAMTS4 is approximately 5ng/lane under non-reducing and reducing conditions.
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG2a
HostGoat
SourceHuman
Concentration~50ug/ml
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 50ug BSA, 5% trehalose. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base, 150mM sodium chloride) containing 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived, recombinant human ADAMTS4 aa213-685.
SpecificityRecognizes human ADAMTS4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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