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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4)

Anti -ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin
motif 4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP N
ADAMTS4 is a secreted member of the ADAM family of proteins. ADAMTS4 is upregulated by TNF-alpha in osteoarthritis and contributes to joint destruction. Its upregulation in some cancers may enhance tumor invasiveness. Human ADAMTS4 consists of a signal peptide (aa1-51), a pro domain (aa52-212) and a mature chain (aa213-837) containing the following domains: catalytic (aa218-425), disintegrin (aa437-519), TSP type-1 (aa520-575), Cys-rich (aa577-685) and spacer (aa686-837). The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine.
Catalog #A0859-60E
ApplicationsSuitable for use in Neutralization, Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsNeutralization: 25ug/ml (IC50) inhibits 50% of the proteolytic activity under conditions in which the enzyme was present at 5ug/ml and the substrate concentration was 100ug/ml. The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with recombinant human Aggrecan G1-IGD-G2 domains as the substrate. Considering the molecular mass of the enzyme (50kD) and the antibody (150kD), IC50 was achieved at ~2:1 molar ratio of the antibody to enzyme.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~2ng/well.
Western Blot: 1-2ug/ml. Using a colormetric system, the detection limit is ~25ng/lane under non-reducing conditions. Use under reducing conditions is not recommended.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human ADAMTS4 from conditioned cell medium.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9H40
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa213-685 from human ADAMTS4 expressed in NSO cells (O75173).
SpecificityRecognizes human ADAMTS4. Does not crossreact with recombinant human ADAMTS1, ADAMTS5, ADAMTS13 or ADAMTSL1.2. Species Sequence Homology: chimpanzee; 100%, canine; 97%, mouse, rat, bovine; 94%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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