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Anti -ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3 motif)
Anti -ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3
motif)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IP N |
|
| Catalog # | A0859-61E4 | | Applications | Suitable for use in Neutralization, ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Immunoprecipitation: 25ug/ml. Sample used was conditioned culture medium spiked with recombinant human ADAMTS5. | | Western Blot: 0.1ug/ml using recombinant human ADAMTS5 as sample | | Neutralization: Measured by its ability to neutralize recombinant human ADAMTS5 (4.3ug/ml) cleavage of recombinant human aggrecan G1IGDG2 domain (100ug/ml). The neutralization dose (ND50) is typically 43ug/ml. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.2mg/ml (after reconstitution) | | Form | Supplied as a powder lyophilized from a sterile-filtered solution in PBS containing 5% trehalose. Reconstitute to 0.2mg/ml with sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | NS0-derived, recombinant human ADAMTS5; aa262 -622. | | Specificity | Recognizes human ADAMTS5. Exhibits <1% cross-reactivity with recombinant human ADAMTS1 in direct ELISA and Western Blot. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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