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A0859-61E4 Goat Anti-ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3 motif)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
Q9UNA0
Isotype
IgG
Grade
Affinity Purified
Applications
E IP WB
Crossreactivity
Hu
Accession #
Q9UNA0
Gene ID
11096
Shipping Temp
Blue Ice
Storage Temp
-20°C

ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as aggrecanase-2 and ADAMTS11, is a member of the family of secreted zinc proteases with a multi-domain structure (1, 2). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. ADAMTS5 is an active protease effectively cleaving alpha 2-macroglobulin (3), aggrecan (4), and brevican (5), and is inhibited by TIMP-3 with inhibition constants in the subnanomolar range (6). Based on the murine model studies (7, 8), this protease may be a key enzyme in the degradation of cartilage leading to osteoarthritis and recombinant human eumatoid arthritis. The purified recombinant human ADAMTS5 starts at the N‑terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of recombinant human ADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat. The aggrecanase activity can be inhibited by 5 mM 1,10-phenanthroline and recombinant human TIMP-3.

Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Immunoprecipitation: 25ug/ml using conditioned culture medium spiked with recombinant human ADAMTS5. Western Blot: 0.1ug/ml using recombinant human ADAMTS5 as sample Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to aa262-622 from human ADAMTS5, expressed in NSO cells (Q9UNA0).
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ADAMTS5. In Western Blot, crossreacts <5% with recombinant human ADAMTS1 and recombinant human ADAMTS1.2.
References
1. Porter, S. et al. (2005) Biochem. J. 386:15. 2. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94. 3.Tortorella, M.D. et al. (2004) J. Biol. Chem. 279:17554. 4. Vankemmelbeke, M.N. et al. (2001) Eur. J. Biochem. 268:1259. 5. Nakada, M. et al. (2005) Acta Neurophathol (Berl). 110:239. 6. Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501. 7. Glasson, S.S. et al. (2005) Nature, 434: 644. 8. Stanton, H. et al. (2005) Nature, 434:648.
USBio References
No references available
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