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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3 motif)

Anti -ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3
motif)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP N
Catalog #A0859-61E4
ApplicationsSuitable for use in Neutralization, ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoprecipitation: 25ug/ml. Sample used was conditioned culture medium spiked with recombinant human ADAMTS5.
Western Blot: 0.1ug/ml using recombinant human ADAMTS5 as sample
Neutralization: Measured by its ability to neutralize recombinant human ADAMTS5 (4.3ug/ml) cleavage of recombinant human aggrecan G1­IGD­G2 domain (100ug/ml). The neutralization dose (ND50) is typically 43ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a powder lyophilized from a sterile-filtered solution in PBS containing 5% trehalose. Reconstitute to 0.2mg/ml with sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived, recombinant human ADAMTS5; aa262 -622.
SpecificityRecognizes human ADAMTS5. Exhibits <1% cross-reactivity with recombinant human ADAMTS1 in direct ELISA and Western Blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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