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You are here:Home » Antibodies » Antibodies-Matrix Metalloproteinases » Anti -ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3 motif)

Anti -ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3
motif)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
ADAMTS5, also known as aggrecanase-2 and ADADMTS11, ADAMTS5 precursor consists of multiple domains. The purified rhADAMTS5 starts at the N-terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of rhADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat.
Catalog #A0859-61E5
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml under non-reducing conditions
Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human ADAMTS5
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9H41
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein A affinity chromatography
ImmunogenRecombinant protein corresponding to aa262- 622 of human ADAMTS5, expressed in NSO cells (Q9UNA0)
SpecificityRecognizes human ADAMTS5. In Western Blot, no cross-reactivity with rhADAMTS1 or rhADAMSTL1.2 is observed.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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