Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAMTS13 (A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13)

Anti -ADAMTS13 (A disintegrin-like and metalloprotease (reprolysin type) with
thrombospondin type 1 motif, 13)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IP
ADAMTS13 (A disintegrin and metalloprotease with TSP motifs 13) is a 140-230kD, secreted glycoprotein member of the ADAMTS family of proteases. ADAMTS13 is produced by the liver and circulates in plasma where it cleaves and reduces the activity of von Willebrand factor. ProADAMTS13 is 1398aa in length. It contains a 45aa prodomain (aa30-74), one peptidase domain (aa80-286) and one disintegrin (aa287-383) domain, multiple TSP regions and two CUB domains. The prodomain does not affect activity, while the TSP and CUB domains appear to be necessary for activity. One truncated form exists that shows truncation after Gln448. Over
aa34-688, human ADAMST13 shares 76% aa identity with mouse ADAMST13.
Applications:
Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Catalog #A0859-61F2
Recommended DilutionELISA: 0.5-1.0ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenCHO cell-derived, recombinant human ADAMTS13 isoform aa34-688.
SpecificityRecognizes human ADAMTS13.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links