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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAMTSL-1 (Punctin Aggrecanase I, A Disintegrin And Metalloproteinase with ThromboSpondin-3 motif)

Anti -ADAMTSL-1 (Punctin Aggrecanase I, A Disintegrin And Metalloproteinase with
ThromboSpondin-3 motif)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP
Produced in goats immunized with purified, NS0-derived, recombinant human Punctin (rhPunctin; aa29-525). Human Punctin specific IgG was purified by human Punctin affinity chromatography. Punctin is a secreted glycoprotein resembling members of the ADAMTS family although it lacks the propeptide region and the metalloproteinase and disintegrin-like domains.
Punctin is expressed in adult skeletal muscle and may have important functions in the extracellular matrix. Alternative splicing of the Punctin transcript results in three isoforms. Isoform 1 is the longest and contains the entire isoform 2. Isoform 3 has the same sequence as isoforms 1 and 2 until residue 361, but a unique C-terminal end of 78 amino acids.
Catalog #A0859-62A2
ApplicationsSuitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.5-1.0ug/ml. The detection limit for rhPunctin is approximately 1ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit for rhPunctin is approximately 2ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively.
Immunoprecipitation: Used to immunoprecipitate rhPunctin from conditioned media of transfected NS0 cells. The recovered rhPunctin can be detected by western blot analysis using a monoclonal antibody.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived, recombinant human Punctin aa29-525.
SpecificityRecognizes human Punctin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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