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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IP IH IF

Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Src homologous and collagen-like (Shc) and epidermal growth factor (EGF) receptor. AP2 is composed of four subunits, two separate 100kD gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17kD subunit. There are two alpha-adaptin genes, alphaA and alphaC which have a tissue specific pattern of expression.
Catalog #	A0859-90
Applications
	Suitable for use in Immunofluorescence, Western Blot Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:100; Recognizes an ~100kD doublet representing the two isoforms of alpha-adaptin from porcine brain extract.
	Immunohistochemistry: paraffin sections
	Immunofluorescence (IC): 1:100; Staining of alpha-adaptin in transfected COS cells yields distinct punctate dots on the plasma membrane.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	4i290
Host	Mouse
Source	Bovine
Concentration	Not determined
Form	Supplied as a liquid in PBS, 0.05% sodium azide.
Purity	Ascites
Immunogen	Purified bovine brain adaptor complexes.
Specificity	Recognizes bovine alpha Adaptin. Detects the products of both alpha-adaptin genes, alpha A and alpha C, as well as an alternatively spliced isoform of alpha A found in neurons.
Species Crossreactivity	Human, rat, mouse, porcine, chicken, primate and amphibians.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.