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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -Adaptin, alpha (AP-2 Complex Subunit alpha-1, 100kD Coated Vesicle Protein A, Adapter-related Protein Complex 2 alpha-1 Subunit, Adaptor Protein Complex AP-2 Subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin Assembly Protein Complex 2 alpha-A Lar

Anti -Adaptin, alpha (AP-2 Complex Subunit alpha-1, 100kD Coated Vesicle Protein A,
Adapter-related Protein Complex 2 alpha-1 Subunit, Adaptor Protein Complex AP-2 Subunit
alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin Assembly Protein Complex 2 alpha-A Lar

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC) and alpha-adaptin A and C. Coat proteins of approximately 100kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
Catalog #A0859-90B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeGG2a
Clone No10K99
HostMouse
SourceBovine
ConcentrationAs reported
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenAP-2 adaptor polypeptides from bovine brain.
SpecificityRecognizes Adaptin, alpha. Species Crossreactivity: human, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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