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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B

Adaptins are important components of heterotetrameric adaptor complexes (APs) whose role is to promote the formation of clathrin-coated pits and vesicles. The AP-1 adaptor, localized at the trans-Golgi network, is composed of 2 approximately 100kD subunits, beta-prime-adaptin and gamma-adaptin. The predicted 825aa protein shares 99% identity with mouse gamma-adaptin. Northern blot analysis revealed that gamma-adaptin was expressed as a 7.5kb transcript in all human tissues tested. Human gamma-adaptin gene (ADTG) is assigned to 16q23.
Catalog #	A0859-94
Application	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 2-4ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20 PBS to1mg/ml Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	10K100
Host	Mouse
Source	Bovine
Concentration	As reported
Form	Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	AP-1 adaptor from bovine brain.
Specificity	Recognizes Adaptin, gamma. Species Crossreactivity: human, rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.