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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IP IH IF N

Catalog #	A0868-35
Applications	Suitable for use in Immunofluorescence microscopy, Immunohistochemistry, Immunoprecipitation, Neutralization Assay, Affinity chromatography and ELISA. Cannot be used for Immunoblot. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:20
	Immunoprecipitation: 1:5
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG3
Clone No	5F148
Host	Mouse
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder in PBS, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Adeno-associated virus capsid proteins and virus particles.
Specificity	Recognizes AAV-2 found in human and monkey in ELISA. Also applicable to AAV-3. Specifically reacts wtih intact AAV particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids not present in denatured capsid proteins and native but unassembled capsid proteins. Mjor reaction attributed to aa369-378 of AAV-2 capsids.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.