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You are here:Home » Antibodies » Antibodies-Infectious Disease » Anti -Adeno-Associated Virus (AAV) VP1, VP2, VP3

Anti -Adeno-Associated Virus (AAV) VP1, VP2, VP3


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Useful for immunolocalization studies of capsid formation in combination with monoclonal A0868-35 and rabbit polyclonal antibody to AAV capsid proteins (Cat. % A0868-36N). Clone B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wo- bus et al., see below) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.
Catalog #A0868-36K
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, and Affinity chromatography. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 1:10 Fix with formaldehyde. Incubation Time 1 hr at RT.
Immunoprecipitation: Mainly free VP proteins.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS or TBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone NoB1
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile PBS or TBS.
PurityPurified by Protein A affinity chromatography.
ImmunogenAdeno-associated virus capsid proteins and virus particles.
SpecificityRecognizeds AAV-2 found in human and monkey. Cross-reactive with AAV-1, AAV-3, AAV-4, AAV-5 and AAV-6 (weak cross-reactivity with AAV-4, AAV-5, AAV-11 and AAV-12).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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