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Anti -Adeno-Associated Virus 2, VP1, VP2, VP3 (AAV2)
Anti -Adeno-Associated Virus 2, VP1, VP2, VP3 (AAV2)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH IF |
|
| Catalog # | A0868-36K | | Applications | Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, and Affinity chromatography. Other applications have not been tested. | | Recommended Dilution | Immunohistochemistry: 1:10 Fix with formaldehyde. Incubation Time 1 hr at RT. | | Immunoprecipitation: Mainly free VP proteins. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding 40-50% glycerol, ddH20. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 5F165 | | Host | Mouse | | Concentration | ~0.05mg/ml | | Form | Supplied as a lyophilized powder in 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml 40-50% glycerol, ddH20. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Adeno-associated virus capsid proteins and virus particles. Epitope: 726 to aa733 (C-terminus; common to all 3 VP proteins). | | Specificity | Recognizeds AAV-2 found in human and monkey, and all published AAV serotypes (AAV-4 only weak cross-reaction). Combination with monoclonal antibody and rabbit polyclonal antibody to AAV capsid proteins. Reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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