| The A1 Adenosine Receptor is involved in smooth muscle contraction, control of heart rate, sedation, and lipolysis. Expression of the adenosine A1 receptor has been reported in the central nervous system, particularly in the cerebral cortex, hippocampus, thalamus, striatum, and cerebellum, and in peripheral tissues including heart, adipose, testis, and kidney. ESTs have been isolated from brain, breast, eye, kidney, salivary gland, and testis libraries. |
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| Catalog # | A0869-52 |
| Applications | Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 1:10,000-1:50,000 |
| Western Blot (ECL): 1-10ug/ml. Use chemiluminescence technique. Higher concentrations of antibody may be necessary if colorimetric detection or milk-based antibody diluent are used. |
| Immunohistochemistry (Frozen): 10-50ug/ml Light fixation with 0.2% picric acid is recommended. |
| Immunocytochemistry: Light 2% PFA fixation for 5-10 minutes is recommended. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 0.1% BSA. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to the 3rd extracellular domain aa163-176, GEPVIKAEFEKVIS, of the rat A1R gene. |
| Specificity | Recognizes rat Adenosine A1 receptor (A1R) at 79 and 39kD bands in cortical membranes. The 79kD band can be converted to 39kD band by agonist or antagonist treatment and could represent A1R dimers. Species Crossreactivity: human, porcine, sheep and guinea pig. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
