| The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, AR s have been classified into A1, A2a, A2b , and A3 . The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various, ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular and the C-termini cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1). |
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| Catalog # | A0869-56E |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-10ug/ml using ECL. |
| ELISA: 1:10,000-50,000 using 50-100ng control peptide/well. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | A0869-56E |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Canine |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | A 30 amino acid sequence as corresponding to canine A2aR gene (1). This region is within the 4th C-terminal intracellular domain. A cysteine has been added at the NH2 terminus for coupling to KLH. |
| Specificity | Recognizes Adenosine A2a Receptor. Species crossreactivity: Human, bovine and porcine A2a receptors. The canine A2aR21 has homology of 43% (13/30 AA) with rat, 80% (24/30 AA) with human and 70% (21/30 AA) with G. pig A2aR sequences (1). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
