| Adenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases. |
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| Catalog # | A0869-59 |
| Applications | Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:1000-1:5000. Detects Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras. |
| Immunohistochemistry: 1ug/ml. Detects Adenosine Receptor A2A in paraformaldehyde-fixed mouse brain striatal sections. Not detected in Adenosine Receptor A2A knockout mice. |
| Flow Cytometry: Detects Adenosine Receptor A2A in transfected HEK293 cells. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Mouse brain striatal sections |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 5H30 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.01M sodium phosphate, pH 7.0, 0.15M sodium chloride, 0.01M EDTA. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | 6His-tagged full-length human recombinant A2A receptor. Epitope: third intracellular loop (SQPLPGER). |
| Specificity | Recognizes human adenosine receptor A2A at ~43kD. |
| Species Crossreactivity | Mouse, rat, rabbit and canine. Crossreactivity with guinea pig also reported. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
