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You are here:Home » Antibodies » Abs to Receptors » Anti -Adenosine Receptor A2A

Anti -Adenosine Receptor A2A

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
Adenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases.
Catalog #A0869-59
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:5000. Detects Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.
Immunohistochemistry: 1ug/ml. Detects Adenosine Receptor A2A in paraformaldehyde-fixed mouse brain striatal sections. Not detected in Adenosine Receptor A2A knockout mice.
Flow Cytometry: Detects Adenosine Receptor A2A in transfected HEK293 cells.
Optimal dilutions to be determined by the researcher.
Positive ControlMouse brain striatal sections
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5H30
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
Immunogen6His-tagged full-length human recombinant A2A receptor (NM_000675). Epitope: third intracellular loop (SQPLPGER).
SpecificityRecognizes human adenosine receptor A2A at ~43kD.
Species CrossreactivityMouse, rat, rabbit and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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