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You are here:Home » Antibodies » Abs to Receptors » Anti -Adenosine A2b Receptor

Anti -Adenosine A2b Receptor

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Adenosine A2b Receptor is a G-protein coupled adenosine receptor. It is an integral, multi-pass membrane protein which activates adenylate cyclase activity in the presence of adenosine.
Catalog #A0869-66
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and ELISA. Not suitable for use in Immunoprecipitation. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Frozen, formalin fixed): 10-50ug/ml.
Western Blot: 1-10ug/ml (ECL), 40-100ug membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
ELISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.
Positive ControlsEpithelial cells of the crypts, Synctiotrophoplast cells of human placenta, T84 human colon adenocarcinoma cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to a 16aa sequence of the 2nd extracellular domain of the human brain A2bR gene.
SpecificityRecognizes human Adenosine A2b receptor. Recognizes a band of ~50-52kD in several rat, mouse and human tissues (thymus, placenta, colon and small intestine). Also recognizes the 35kD band in small intestine. Does not recognize the native receptors. No bands were detected in skeletal muscle or heart. Will not recognize native protein in immunoprecipitation experiments. Species Crossreactivity: rat and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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