Suitable for use in Immunohistochemistry, Western Blot and ELISA. Not suitable for use in Immunoprecipitation. Other applications have not been tested.

Immunohistochemistry (Frozen, formalin fixed): 10-50ug/ml. Western Blot: 1-10ug/ml (ECL), 40-100ug membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used. ELISA: 1:10,000-1:50,000 Optimal dilutions to be determined by the researcher.

Epithelial cells of the crypts, Synctiotrophoplast cells of human placenta, T84 human colon adenocarcinoma cells.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Synthetic peptide corresponding to a 16aa sequence of the 2nd extracellular domain of the human brain A2bR gene.

Supplied as a liquid in PBS, 0.1% BSA.

Purified by affinity chromatography.

Recognizes human Adenosine A2b receptor. Recognizes a band of ~50-52kD in several rat, mouse and human tissues (thymus, placenta, colon and small intestine). Also recognizes the 35kD band in small intestine. Does not recognize the native receptors. No bands were detected in skeletal muscle or heart. Will not recognize native protein in immunoprecipitation experiments. Species Crossreactivity: rat and mouse