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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B

The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b, and A3. The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various, ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular and the C-termini cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1).
Catalog #	A0869-66A
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-1:5000 using ECL.
	ELISA: 1:10,000-1:50,000 using 50-100ng A1R control peptide/well.
	Optimal dilutions to be determined by the researcher.
Control Peptide	A0869-66G
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Purity	Serum
Immunogen	A 16 amino acid sequence corresponding to human brain A2bR gene (1). This region corresponds to the 2nd extracellular mid-region domain. Coupled to KLH.
Specificity	Recognizes human Adenosine A2b Receptor. Species Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.