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Specifications

Catalog #	A0876-35
Source	HeLa cells, inoculated with Adenovirus, type 6, strain Tonsil 99
Preparation	Virus was grown on monolayer of HeLa cells. When the cultures showed pronounced c.p.e. virus containing medium was purified from cell fragments by low speed centrifugation (4,000 rpm 40 min.). Virus was pelleted by high speed centrifugation at 18,000 rpm for 2.5 hours in the SW27 rotor for 20 min. at 4°C, then the supernatant was collected.
Inactivation	Thimerosal and beta-propiolactone
Applications	Specific methodologies have not been tested using this product.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Storage and Stability	Aliquot and store at -20°C or lower. Avoid multiple freez-thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note	No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.
Source	HeLa cells
Purity	90% by SDS-PAGE.
Concentration	~1mg/ml (Lowry method)
Form	Supplied as a liquid in 0.05M Tris-HCl, pH 8.0, 0.1M sodium chloride, 5mMEDTA, 0.1% sodium azide, 0.005% tthimerosal.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.