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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH

Adenoviruses are non-enveloped viruses with a linear double-stranded DNA genome belonging to the Adenoviridae family. The virion also has a unique spike or fibre associated with each penton base of the capsid that aids in attachment to the host cell via the coxsackie-adenovirus receptor on the surface of the host cell. They are commonly used as vectors for in vitro as well as in vivo gene transfer to a number of different tissues. There are 51 identified serotypes of human adenovirus, divided into six species (A-F), each with a different tropism. The most commonly used adenovirus vector systems are based on adenovirus serotypes 2 and 5 (Ad2 and Ad5), both belonging to species C. Most infections with adenovirus result in infections of the upper respiratory tract. Adenovirus infections also show up as conjunctivitis, tonsilitis viral meningitis or encephalitis, bronchiolitis or pneumonia.
Catalog #	A0876-48A
	The adenovirus early gene products E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kD) with multifunctional capabilities and forms a specific complex with the retinoblastoma tumor suppressor gene product. The E1a and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region.
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (formalin fixed paraffin embedded): 1:50-1:75
	Optimal dilutions to be determined by the researcher.
Positive Control	Adenovirus infected tissue
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	11E10
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a liquid, 0.05% sodium azide.
Purity	Purified
Immunogen	Adenovirus. Cellular Localization: Nuclear.
Specificity	Recognizes human Adenovirus.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.