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You are here:Home » Antibodies » Abs to Infectious Disease Adenovirus » Anti -Adenovirus (Biotin)

Anti -Adenovirus (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified E B IH IF
The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kD) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
Catalog #A0876-50H
ApplicationsSuitable for use in Immunohistochemistry, ELISA, Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:8000
ELISA: 1:5000
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~4-5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added. Labeled with Biotin.
PurityPurified IgG fraction to a purity of > 95%.
ImmunogenPurified, disrupted Adenovirus Type 5 virions.
SpecificityRecognizes numerous Adenovirus proteins, including hexon. Does not crossreact with Para 1-3, Infl. A & B ,RSV, hMPV or Rhinovirus (type 16). Uninfected Cell Reactivity: Negative vs. HE-p2, LLCMK2 and Vero cells by indirect immunofluorescence. Reactive with types 2, 3, 4, 5 & 6. Broad reactivity with most/all Adeno types expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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