USBio Logo

A0879-05 Mouse Anti-Adenovirus, Types 2,5, Early Region (E1A)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Swiss Prot
P03254, P03255
Isotype
IgG2a,k
Clone Number
4i25 (M73)
Grade
Affinity Purified
Applications
IC IP WB
Accession #
NP_040507
Shipping Temp
Blue Ice
Storage Temp
-20°C

The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.

Applications
Suitable for use in Immunoprecipitation, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 0.05-0.1ug/ml detects the Ad2 and Ad5 E1A gene products in RIPA lysates from HEK293 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0879-05 (0.1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Immunoprecipitation: An independent laboratory has reported A0879-05 immunoprecipitates Ad2 and Ad5 E1A gene products and associated proteins. Immunocytochemistry: An independent laboratory has reported thatA0879-05 immunostains Ad2 and Ad5 E1A gene products. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
TrpE-Ad2 E1A fusion protein isolated from E. coli.
Form
Supplied as a liquid in 0.05M sodium phosphate, pH 7.4, 0.2% gelatin, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the three adenovirus (Ad) 2 and 5 E1A gene products, Mr 36-50kD.
References
1. Harlow, E., et al., (1985). J. Virol. 55: 533-46, 1985. 2. Nowling, T. et al., (2003). J Biol Chem 278:13696-705.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved