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Anti -Adenovirus, Type 2 E1A (BSA & Azide Free)
Anti -Adenovirus, Type 2 E1A (BSA & Azide Free)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Highly Purified |
B IP IH IF |
|
| The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen. | | | Catalog # | A0879X | | Applications | Suitable for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Native verified) and Western Blot. Other applications not tested. | | Recommended Dilution | Immunoprecipitation: Use Protein A; 2ug antibody/mg protein lysate | | Western Blotting: 1ug/ml, 2hrs, RT | | Immunohistochemistry: 2-4ug/ml, 30min., RT. Staining of formalin-fixed tissues requires boiling sections in 10mM citrate buffer, pH 6.0. for 10-20min., followed by cooling at RT for 20min. | | Optimal dilutions to be determined by the researcher. | | Molecular Weight of Antigen | 35-46kD | | Positive Control | 293 cells or adenovirus-infected tissue | | Cellular Localization | Nuclear | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | 0.N.7 | | Host | Mouse | | Concentration | 1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See A0879X. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Adenovirus | | Specificity | Species Reactivity: Adenovirus. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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