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You are here:Home » Molecular Biology » MB-Infectious Disease » ADENOVIRUS TYPE 5 SOLUBLE ANTIGEN

ADENOVIRUS TYPE 5 SOLUBLE ANTIGEN

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Specifications

Adenovirus 5 belongs to Adenoviridae, a family of viruses with at least 51 serotypes that cause a variety of illnesses, mostly being upper respiratory tract infections. Adenovirus type 5 antigen is collected from both the cell and supernatant phases of tissue culture. The antigen is harvested by disrupting optimally infected monolayers in tissue culture medium. Cellular debris is removed by centrifugation.
Catalog #A0880-03K
The antigen preparation is inactivated by heat treatment at 54°C for 2 hours. Inactivity is confirmed by growth on 293 cells for two weeks with no CPE seen.
SourceInactivated adenovirus type 5
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at-20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceInactivated adenovirus type 5
PurityPurified by centrifugation.
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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