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You are here:Home » Antibodies » Abs to Infectious Disease Adenovirus » Anti -Adenovirus, Type 5, Early Region (E1A)

Anti -Adenovirus, Type 5, Early Region (E1A)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kD) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
Catalog #A0880-05
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunofluorescence: 0.2-0.4ug/ml
Immunoprecipitation (Native): 2ug/mg protein lysate. Use Protein A.
Western Blot: 0.5-1ug/ml for 2 hours at RT
Immunohistochemistry: 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, 10-20 minutes, followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.
Positive Control293 cells or adenovirus infected cells and tissues.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No0.N.8
HostMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See A0880-05X.
PurityPurified by Protein A affinity chromatography.
ImmunogenAdenovirus. Cellular Localization
SpecificityRecognizes Adenovirus
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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