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You are here:Home » Antibodies » Abs to Infectious Disease Adenovirus » Anti -Adenovirus Fiber (Monomer and Trimer) (BSA & Azide Free)

Anti -Adenovirus Fiber (Monomer and Trimer) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IF
Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the typespecific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron.
Catalog #A0880-19AX
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Positive ControlAdenovirus infected cells and tissues.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a, k
Clone No3F13
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See A0880-19A
PurityPurified by Protein A affinity chromatography.
ImmunogenUV-irradiated Ad2 virus. MW of Antigen
SpecificityRecognizes both monomers and trimers, as judged by reactivity with both boiled (monomers (62kD) only) and unboiled (both monomers and trimers (180200kD bands)) fiber on Western blots and by indirect immunofluorescence studies of cells infected with a temperature-sensitive Ad5 fiber mutant (H5ts142) which does not form trimers at the nonpermissive temperature at both 320C and 39.50C.(1) Species Crossreactivity: Adenovirus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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