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You are here:Home » Antibodies » Abs to Proteins » Anti -Adipophilin (ADFP, Adipose Differentiation-related Protein)

Anti -Adipophilin (ADFP, Adipose Differentiation-related Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase.
ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
Catalog #A0887-01A
ApplicationsSuitable for use in Western Blot, Immunofluorescence, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Immunohistochemistry (Paraffin): 1:200. Staining was observed on the membrane of mouse liver cells. Antigen retrievak with sodium citrate buffer, pH 6.0 is recommended prior to staining paraffin tissues.
Immunofluorescence (IC): 1:50-1:200 Membrane staining was observed in HeLa cells
Optimal dilutions to be determined by the researcher.
Positive Control Mouse and human liver lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 30% glycerol, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide made to the C-terminal region within residues 350-425 of the mouse protein (Swiss-Prot# P43883). Cellular Localization: Membrane.
SpecificityRecognizes mouse Adipophilin at ~51kD. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesADRP


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