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You are here:Home » Antibodies » Abs to Proteins » Anti -Adipophilin (ADFP, Adipose Differentiation-related Protein) (FITC)

Anti -Adipophilin (ADFP, Adipose Differentiation-related Protein) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IC FC
Adipophilin / ADRP is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al.; for review see e.g. Targett- Adams et al.); inducible by etomoxir. Enhanced expression of Adipophilin / ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry of e.g. HCV via lipid droplets (see e.g. Hope et al.).
Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir.
Catalog #A0887-07
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen): 1:10-1:20 in PBS, pH 7.4; Incubation Time: 1 hour at RT or overnight at 4°C.
Immunohistochemistry: Paraffin-embedded tissue requires microwave treatment.
Optimal dilutions to be determined by the researcher.
Reactivity on tested Cultured Cell LinesCaco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelia), RV, PC-12 (rat adrenal gland), MDCK; negative with glioma.
Storage and StabilityStore product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. 
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F158
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, 0.5% BSA, 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa5-27 from the N-terminus of human Adipophilin.
SpecificityRecognizes human adipophilin. Species Crossreactivity: rat, canine. Does not crossreact with bovine or mouse.
Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stains lipid-storing CD68-positive macrophages.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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