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You are here:Home » Molecular Biology » MB-Proteins » Adipophilin, Recombinant, Human (ADFP, Adipose Differentiation-related Protein)

Adipophilin, Recombinant, Human (ADFP, Adipose Differentiation-related Protein)

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Specifications

Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation. Recombinant Human ADRP produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-437 and having a total molecular mass of 50.2kD. rhADFP contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.
Catalog #A0887-10
ApplicationsSuitable for use in ELISA, Western Blot and Inhibition Assays. Other applications not tested.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight49kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from 20mM Tris, pH 7.5 and 20mM sodium chloride. Reconstitute to 0.5mg/ml with ddH2O; let pellet dissolve completely, and sterile filter.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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