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Adipophilin, Recombinant, Human (ADFP, Adipose Differentiation-related Protein)
Adipophilin, Recombinant, Human (ADFP, Adipose Differentiation-related Protein)
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| Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation. Recombinant Human ADRP produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-437 and having a total molecular mass of 50.2kD. rhADFP contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques. | | | Catalog # | A0887-10 | | Applications | Suitable for use in ELISA, Western Blot and Inhibition Assays. Other applications not tested. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute with ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 49kD | | Source | E. coli | | Purity | 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. | | Concentration | ~0.1mg/ml | | Form | Supplied as a lyophilized powder from 20mM Tris, pH 7.5 and 20mM sodium chloride. Reconstitute to 0.5mg/ml with ddH2O; let pellet dissolve completely, and sterile filter. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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