Adiponectin

Clone Type

Polyclonal

Host

Rabbit

Source

Mouse

Swiss Prot

Q60994

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue. Full-length adiponectin was expressed in HEK293 cells stably transfected with pcdm-F vector encoding FLAG epitope-tagged mouse adiponectin. Recombinant adiponectin was purified using anti-FLAG M2 affinity gel (Sigma-Aldrich) and was eluted with FLAG peptide. FLAG peptides were removed by an extensive wash with an excessive volume of saline. Highly sensitive matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass-spectrometric analysis confirmed that FLAG peptide was not detectable in the protein solution.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

Indirect ELISA: >1:100,000 using 25ng of antigen coated per well. Optimal dilutions to be determined by the researcher.

Storage and Stability

Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

Recombinant Murine Adiponectin produced in HEK293 (Human embryonic kidney cell line).

Form

Supplied as a lyophilized powder in PBS, pH 7.2

Purity

Purified by immunoaffinity chromatography.

Specificity

The recombinant mouse adiponectin is 100% homologous with the Mouse serum adiponectin.

References

1. Xu A, Wang Y, Keshaw H, Xu LY, Lam KS, Cooper GJ. The fat-derived hormone adiponectin alleviates alcoholic and nonalcoholic fatty liver diseases in mice. J Clin Invest. 2003;112:91-100. 2. Wang Y, Xu A, Knight C, Xu LY, Cooper GJ. Hydroxylation and glycosylation of the four conserved lysine residues in the collagenous domain of adiponectin. Potential role in the modulation of its insulin-sensitizing activity. J Biol Chem. 2002;277:19521-19529. 3. Yamauchi T, Kamon J, Minokoshi Y, Ito Y, Waki H, Uchida S, Yamashita S, Noda M, Kita S, Ueki K, Eto K, Akanuma Y, Froguel P, Foufelle F, Ferre P, Carling D, Kimura S, Nagai R, Kahn BB, Kadowaki T. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMPactivated protein kinase. Nat Med. 2002;8:1288-1295. 4. Berg AH, Combs TP, Du X, Brownlee M, Scherer PE. The adipocyte-secreted protein Acrp30 enhances hepatic insulin action. Nat Med. 2001;7:947-953.

USBio References

No references available

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A0891 Rabbit Anti-Adiponectin (Acrp30, Adipocyte Complement-related Protein of 30kD, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Clone Type

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Swiss Prot

Isotype

Grade

Applications

Crossreactivity

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References

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