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You are here:Home » Antibodies » Antibodies-Obesity Proteins » Anti -Adiponectin (Acrp30, Adipocyte Complement-related Protein of 30kD, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Anti -Adiponectin (Acrp30, Adipocyte Complement-related Protein of 30kD, AdipoQ, apM1,
Gelatin Binding Protein 28, GPB28)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue. Full-length adiponectin was expressed in HEK293 cells stably transfected with pcdm-F vector encoding FLAG epitope-tagged mouse adiponectin. Recombinant adiponectin was purified using anti-FLAG M2 affinity gel (Sigma-Aldrich) and was eluted with FLAG peptide. FLAG peptides were removed by an extensive wash with an excessive volume of saline. Highly sensitive matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass-spectrometric analysis confirmed that FLAG peptide was not detectable in the protein solution.
Catalog #A0891
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionIndirect ELISA: >1:100,000 using 25ng of antigen coated per well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilized powder in PBS, pH 7.2
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant Murine Adiponectin produced in HEK293 (Human embryonic kidney cell line).
SpecificityThe recombinant mouse adiponectin is 100% homologous with the Mouse serum adiponectin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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