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Anti -Adiponectin (Acrp30, Adipocyte Complement-related Protein of 30kD, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)
Anti -Adiponectin (Acrp30, Adipocyte Complement-related Protein of 30kD, AdipoQ, apM1,
Gelatin Binding Protein 28, GPB28)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue. Full-length adiponectin was expressed in HEK293 cells stably transfected with pcdm-F vector encoding FLAG epitope-tagged mouse adiponectin. Recombinant adiponectin was purified using anti-FLAG M2 affinity gel (Sigma-Aldrich) and was eluted with FLAG peptide. FLAG peptides were removed by an extensive wash with an excessive volume of saline. Highly sensitive matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass-spectrometric analysis confirmed that FLAG peptide was not detectable in the protein solution. | | | Catalog # | A0891 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Indirect ELISA: >1:100,000 using 25ng of antigen coated per well. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder in PBS, pH 7.2 | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant Murine Adiponectin produced in HEK293 (Human embryonic kidney cell line). | | Specificity | The recombinant mouse adiponectin is 100% homologous with the Mouse serum adiponectin. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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