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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -ADPN (Adiponutrin, Patatin-like Phospholipase Domain containing 3)

Anti -ADPN (Adiponutrin, Patatin-like Phospholipase Domain containing 3)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
Human ADPN (Adiponutrin; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53kD member of the PNPLA family of phospholipase A2 enzymes. It is expressed in white adipocytes. Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways. ADPN is likely to be a type II transmembrane protein that is 481aa in length. It contains one patatin domain (aa10-179) that
is associated with in vitro enzyme activity and a transmembrane segment between
aa42-62. There appear to be two splice variants. One shows a four aa deletion of aa59-62 and a second shows an alternate start site at Met379. Over aa 160-349, human ADPN shares 61% aa identity with mouse Adiponutrin.
Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Catalog #A0901-25A
Recommended DilutionELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Immunohistochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant human ADPN, aa160-349 (E. coli).
SpecificityRecognizes human ADPN.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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