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You are here:Home » Kits and Assays » Kits for Apoptosis, BioAssay™ » ADP/ATP Ratio BioAssay™ Kit

ADP/ATP Ratio BioAssay™ Kit

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Specifications

The changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis. The ADP/ATP Ratio Assay kit utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive (detects 100 mammalian cells/well).
Catalog #A0901-40
Kit ComponentsNucleotide Releasing Buffer 1x50ml
ATP Monitoring Enzyme (green cap) 1x1vial
ADP Converting Enzyme (blue cap) 1x1vial
Enzyme Reconstitution Buffer (red cap) 1x450ul.
Storage and StabilityStore the kit at -70°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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