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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IH FC

ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARFs family which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) is localizes in the cytoplasm while the active form (ARF1-GTP) is localized in the membrane (2-3). Active ARF1 also localized to the Golgi complex, regulates the assembly of coat proteins onto membranes and mediates traffic in the ER-Golgi. ATF1 activates phospholipase D and phosphatidyllino- sitol 4-phosphate 5-kinase and recruits COPI to membranes (4-5).
Catalog #	A0901-80A
Applications	Suitable for use in Flow Cytometry, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:80.
	Western Blot: 1:2000.
	Immunohistochemistry: 1:250-500.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	6k12
Host	Rabbit
Source	Human
Concentration	Not determined.
Form	Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues in human ARF1 (UniProt ID: P84077)
Specificity	Recognizes human ARF1 at 21kD. Predicted to detect splice isoforms 3, 4, 5 based on sequence analysis.
Species Crossreactivity	Mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.