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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -ADP Ribosylation Factor 1 (ARF1)

Anti -ADP Ribosylation Factor 1 (ARF1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH FC
ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARFs family which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) is localizes in the cytoplasm while the active form (ARF1-GTP) is localized in the membrane (2-3). Active ARF1 also localized to the Golgi complex, regulates the assembly of coat proteins onto membranes and mediates traffic in the ER-Golgi. ATF1 activates phospholipase D and phosphatidyllino- sitol 4-phosphate 5-kinase and recruits COPI to membranes (4-5).
Catalog #A0901-80A
ApplicationsSuitable for use in Flow Cytometry, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:80.
Western Blot: 1:2000.
Immunohistochemistry: 1:250-500.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k12
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues in human ARF1 (UniProt ID: P84077)
SpecificityRecognizes human ARF1 at 21kD. Predicted to detect splice isoforms 3, 4, 5 based on sequence analysis.
Species CrossreactivityMouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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