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Anti -ADP Ribosylation Factor 1 (ARF1, DJ133P16.1)
Anti -ADP Ribosylation Factor 1 (ARF1, DJ133P16.1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. | | | Catalog # | A0901-80E | | Positive Control | HepG2 cell lysate | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:8000. | | Western Blot: 1-3ug/ml. ~ 18kD band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7kD according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1). | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | | | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris-saline, pH7.2, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide, C-EGLDWLSNQLRNQK, representing the C Terminus of the human protein according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1. Homology | | Specificity | Species Crossreactivity: Human. Other species not tested. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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