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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -ADP Ribosylation Factor 1 (ARF1, DJ133P16.1)

Anti -ADP Ribosylation Factor 1 (ARF1, DJ133P16.1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Catalog #A0901-80E
Positive Control HepG2 cell lysate
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:8000.
Western Blot: 1-3ug/ml. ~ 18kD band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7kD according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1).
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris-saline, pH7.2, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide, C-EGLDWLSNQLRNQK, representing the C Terminus of the human protein according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1. Homology
SpecificitySpecies Crossreactivity: Human. Other species not tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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