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You are here:Home » Antibodies » Antibodies-Transport Proteins » Anti -ADP Ribosylation Factor 1 (ARF1, DJ133P16.1)

Anti -ADP Ribosylation Factor 1 (ARF1, DJ133P16.1)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARFs family which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) is localizes in the cytoplasm while the active form (ARF1-GTP) is localized in the membrane (2-3). Active ARF1 also localized to the Golgi complex, regulates the assembly of coat proteins onto membranes and mediates traffic in the ER-Golgi. ATF1 activates phospholipase D and phosphatidyllino- sitol 4-phosphate 5-kinase and recruits COPI to membranes (4-5).
Catalog #A0901-80G
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:8000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide of ARF1.
SpecificityRecognizes ARF1 at ~18kD in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7kD according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1). This antibody is expected to recognize all four reported isoforms. Species Crossreactivity: Canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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