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ARF1, Recombinant, Human (ADP-ribosylation Factor 1)
ARF1, Recombinant, Human (ADP-ribosylation Factor 1)
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| ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. It is essential and ubiquitous in eukaryotes, as this protein is involved in vesicular transport and functioning via phospholipase D activation. ARF1 plays a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. | | | Catalog # | A0901-81 | | Source | Recombinant corresponding to aa1-181 of human ARF1 protein, fused to His-tag at N-terminus, expressed in E.coli. | | AA Sequence | MGSSHHHHHH SSGLVPRGSH MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K | | Molecular Weight | 22.8kD (201aa) confirmed by MALDI-TOF | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 22.8kD | | Source | E.coli | | Purity | ~90% (SDS-PAGE). | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20% glycerol, 0.1M sodium chloride, 1mM DTT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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