You are here:Home
»
Molecular Biology
»
MB-Transport Proteins
»
ARF5, Recombinant, Human, aa1-180, His-tag (ADP-ribosylation factor 5)
ARF5, Recombinant, Human, aa1-180, His-tag (ADP-ribosylation factor 5)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| ADP-ribosylation factor 5, also known as ARF5, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization and WASP/Arp2/3 complexes. ARF5 is involved in vesicular transport and functioning via phospholipase D activation. Purified by using conventional chromatography techniques. | | | Catalog # | A0901-86 | | Source | Recombinant corresponding to aa1-180 of human ARF5, fused to His-tag at N-terminal, expressed in E.coli. | | AA Sequence | MGSSHHHHHH SSGLVPRGSH MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA | | TQGTGLYDGL DWLSHELSKR | | Molecular Weight | ~22.6kD (200aa), confirmed by MALDI-TOF | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~22.6kD | | Source | E.coli | | Purity | ~90% (SDS-PAGE) | | Concentration | ~1mg/ml | | Form | Supplied as a liquid. In 20mM Tris-HCl buffer, pH 8, 1mM DTT, 0.1M sodium chloride, 20% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
