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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IP IH

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21kD proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.
Catalog #	A0902-10A
Applications	Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation.
Recommended Dilution	Immunohistochemistry: 1:500
	Western Blot: 1:500
	Note: Not suitable for neutralizing ARF in solution.
Cellular Localization	Golgi apparatus
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6D105
Host	Mouse
Source	Human
Concentration	Not determined
Form	Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
Purity	Ascites
Immunogen	Recombinant human ARF1 (ADP-ribosylation factor-1).
Specificity	Recognizes human, mouse, rat, dog and hamster ARF1, ARF3, ARF5 and ARF6. Detects a band of ~21kD by Western blot.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.