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You are here:Home » Antibodies » Abs to Receptors » Anti -Adrenergic Receptor, Alpha 2a (A2aAR)

Anti -Adrenergic Receptor, Alpha 2a (A2aAR)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH IF
Alpha 2A, 2B, and 2C adrenergic receptors are seven transmembrane receptors that couple to inhibitory G-proteins. Alpha 2C has a wide distribution pattern in the CNS. On neurons alpha 2 adrenergic receptors are located on both pre- and postsynaptic domains where they mediate an inhibitory role in the central and peripheral nervous system. Alpha 2C has a wide distribution pattern in the CNS. At the spinal cord level the vast majority of alpha 2C is not of primary afferent origin; however, a small fraction of neurons in DRG do express mRNA for alpha 2C and harbor the protein. Colocalization has been observed between alpha 2C and enkephalin in the spinal cord dorsal horn, suggesting that alpha 2C may be expressed by a subset of spinal interneurons. The distribution pattern of alpha 2C suggests functions at several levels in the brain. Antibody showed positive staining in rat spinal cord dorsal horn, ventral horn motor neurons and retina. When examined in other brain regions known to express alpha 2A staining was not observed.
Catalog #A0906-64R
ApplicationsSuitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:100-1:500; Antiserum was used on perfusion fixed tissue. Perfusion: 1) Calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.
Slide mounted tissue sections were processed for indirect immunoflourescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4°C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and cover-slipped. Staining was examined using fluorescence microscopy.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid.
PurityPurified by immunoaffinity chromatography
Immunogen(C)KASRWRGRGNREKR; Cysteine added for conjugation
SpecificityRecognizes rat alpha 2a Adrenergic Receptor. Shows positive staining in rat spinal cord dorsal horn, ventral horn motor neurons and retina. When examined in other brain regions known to express alpha 2a, staining was negative. Species Crossreactivity: Mouse and human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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