| ADRB3 is a transmembrane protein and member of the G-protein coupled receptor 1 family primarily expressed by adipose tissue, which acts as a regulator of lipolysis and heat production. The ADRB3 variant Arg-64 has been identified as a possible candidate gene linked with obesity, and to an increased susceptibility to non-insulin-dependent diabetes mellitus (NIDDM). |
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| Catalog # | A0906-91C |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | ELISA: 1:128,000 |
| Western Blot: 0.03-0.1ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-28V IgG, Fc (HRP) Pab RbxGt |
| Recommended Useful Reagents | HUMAN BRAIN TOTAL PROTEIN LYSATE |
| HUMAN CEREBELLUM, LEFT TOTAL PROTEIN LYSATE |
| HUMAN CEREBELLUM, RIGHT TOTAL PROTEIN LYSATE |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% Sodium Azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide sequence PHENSSLAPWPD-C from the N-Terminal region of human ADRB3. |
| Specificity | Recognizes an epitope within the NT region of human Beta-3 adrenergic receptor, otherwise known as ADRB3 in human brain cell lysates at ~ 45kD. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
