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You are here:Home » Antibodies » Antibodies-G Protein Receptors » Anti -Adrenergic Receptor Kinase, beta 1 (bARK1, B1 AR, ARK1 beta, G-Protein Coupled Receptor Kinase 2, GPCR K2, GRK2)

Anti -Adrenergic Receptor Kinase, beta 1 (bARK1, B1 AR, ARK1 beta, G-Protein Coupled
Receptor Kinase 2, GPCR K2, GRK2)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IF
G protein-coupled receptor kinases, a more recently discovered family, have the unique features of phosphorylating G-protein coupled receptors only when they are in their active or stimulated conformations. The range of signals acting through G protein-coupled receptors includes numerous neurotransmitters, chemoattractants, hormones, cytokines, and sensory stimuli such as photons or odorants. Structurally, the G-protein coupled receptor kinases show significant amino acid similarity to other kinases only in the centrally located catalytic domain of about 240 amino acid residues. Rapid phosphorylation of many G protein coupled receptors accompanies stimulus-driven desensitization. There are two types of kinases known to mediate these modifications: second messenger kinases and novel G protein-coupled receptor kinases. G protein-coupled receptor kinase family members include rhodopsin kinase, two isoforms of beta-adrenergic receptor kinase (betaARK), a gene cloned from the Huntington’s disease locus on chromosome 4, GRK5, and several homologs from Drosophila. BetaARK1 is widely distributed throughout the mammalian central nervous system, especially at synapses, suggesting broader roles in regulating a wide variety of neurotransmitter receptors. BetaARK1 was identified as a kinase that phosphorylates betaARs in an agonist-dependent manner. Beta ARK1 has properties similar to the rhodopsin kinase in that BetaARK is also insensitive to cAMP, cGMP, and Ca2+ and does not phosphorylate histone. BetaARK is capable of phosphorylating photoexcited rhodopsin, although the betaAR is a better substrate for betaARK than rhodopsin. Both rhodopsin kinase and betaARK are inhibited by salts such as 0.1M NaCl, low concentrations of Zn (0.1-1mM), detergents such as digitonin, and polyanions such as dextransulfate and polyaspartic acid. Both kinases use ATP as the preferred substrate over GTP. In the case of betaARK, the kinase interacts with G protein beta-gamma subunits as well as with receptors, and the ternary complex of agonist bound receptor, kinase, and beta-gamma subunits is considered to be an active form for the phosphorylation of receptors. Multiple independent lines of evidence indicate that the phosphorylation of receptors by G-protein coupled receptor kinases in vivo regulates their function.
Catalog #A0906-95C
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 3-10ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1-5ug/ml
Immunohistochemistry: 10ug/ml
Immunoprecipitation: 10ug/IP reaction
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3G3
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant b-adrenergic receptor kinase.
SpecificityReacts with mammalian b-adrenergic receptor kinase.
Species CrossreactivityHuman and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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