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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » ADP Colorimetric/Fluorometric BioAssay™ Kit

ADP Colorimetric/Fluorometric BioAssay™ Kit

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Specifications

ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories.
Catalog #A0911
ADP BioAssay™ Kit provides an convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (Lmax = 570nm) or fluorometric method (Ex/Em 535/587nm). The assay is simple, sensitive, stable and high- throughput adaptable. The assay can detect as low as 1uM ADP in biological samples.
Kit Components1. ADP Assay Buffer: 1x25ml
2. ADP Probe (lyophilized): 1x1vial
3. DMSO (anhydrous): 1x0.4ml
4. ADP Converter: 1x1vial
5. ADP Developer Mix: 1x1vial
6. ADP Standard (1umol lyophilized): 1x1vial
Storage and StabilityStore kit at -20°C. Protect from light. Allow Assay Buffer to warm to room temperature
before use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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