| Catalog # | A0921-80 |
| Description | Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop- tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ |
| transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co- repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a |
| mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES |
| and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the 5 1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the 5 1 integrin (4). |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-2ug’ml |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | 293 cell lysate |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide. |
|
| Purity | Purified by immunoaffinity chromatography. |
|
| Immunogen | Synthetic peptide AES antibody was raised against a 16 |
| amino acid peptide from near the carboxy terminus of |
| human AES (Genbank accession No. NP_945320). |
| Specificity | Recognizes human. Species Crossreactivity: mouse and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
