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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -AES, NT (Amino-terminal Enhancer of Split, GRG, ESP1, TLE5)

Anti -AES, NT (Amino-terminal Enhancer of Split, GRG, ESP1, TLE5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #A0921-81
DescriptionAdhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop- tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/
transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co- repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a
mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES
and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the 5 1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the 5 1 integrin (4).
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2-4ug’ml
Optimal dilutions to be determined by the researcher.
Positive Control293 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide AES antibody was raised against a 16
amino acid peptide from near the amino-terminus of
human AES (Genbank accession No. NP_945320)
SpecificityRecognizes human AES. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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