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You are here:Home » Antibodies » Abs to Disease Markers » Anti -AF9, ID (V422) (Protein AF-9, ALL1-fused Gene From Chromosome 9 Protein, Myeloid/Lymphoid Or Mixed-lineage Leukemia Translocated To Chromosome 3 Protein, YEATS Domain-containing Protein, MLLT3, YEATS3)

Anti -AF9, ID (V422) (Protein AF-9, ALL1-fused Gene From Chromosome 9 Protein,
Myeloid/Lymphoid Or Mixed-lineage Leukemia Translocated To Chromosome 3 Protein, YEATS
Domain-containing Protein, MLLT3, YEATS3)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B
The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9;11)(p22;q23). The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified; BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed.
Catalog #A0923-55D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenSynthetic peptide selected from the center region of human AF9 (KLH).
SpecificityRecognizes human AF9.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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