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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -AFAP (actin filament associated protein 1, actin filament associated protein, AFAP, AFAP-110, FLJ56849)

Anti -AFAP (actin filament associated protein 1, actin filament associated protein, AFAP,
AFAP-110, FLJ56849)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
AFAP (actin filament associated protein; also known as AFAP-110) is an adaptor protein that regulates actin filament integrity. It is both multimodular and multimeric, and is known to simultaneously bind actin and cytosolic kinases. As a homomultimer, AFAP binds and separates actin filaments, preventing actin cross-linking. Upon binding to kinases, AFAP is destabilized, promoting actin polymerization. Human AFAP is 730aa in length, and contains SH3 and SH2 binding motifs (aa69-74 and aa91-96), two pleckstrin homology domains (aa153-248 and
aa347-450) and a Leu-zipper/actin binding region (aa511-637). Over aa450-730, human AFAP is 86% identical to mouse and rat AFAP.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Catalog #A0923-75
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant human AFAP aa450-730 (E. coli).
SpecificityRecognizes human AFAP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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