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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

The Forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX and FKHRL1) have sequence similarity to the nematode homologue DAF- 16, which has been shown to mediate signaling via a pathway involving IGFR1, PI3K and Akt. There are three Akt phosphorylation sites in the FKHR proteins: Thr24, Ser256 and Ser319. Phosphorylation of FKHR family members at these sites by Akt promotes cell survival and regulates the cell cycle. Phosphorylation regulates FKHR nuclear translocation and target gene transcription.
Catalog #	A0928
Applications	Suitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:1000
	Optimal dilutions to be determined by the researcher.
Molecular Weight	65kD
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser193 of human AFX.
Specificity	Phospho-AFX (Ser193) Antibody detects endogenous levels of AFX only when phosphorylated at Ser193. Recognizes human, hampster.
Important Note	This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.