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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -AFX, phosphorylated (Ser197)

Anti -AFX, phosphorylated (Ser197)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Several studies indicate that FKHR and AFX, mammalian homologues of the Caenorhabditis elegans forkhead transcription factor DAF-16, function in the insulin signaling pathway promoter contains one IRS, while in the IGFBP-1 promoter two copies of IRS are arranged as an inverted palindrome. Three tandem copies of IRSs are present in the G6Pase. AFX has been recently shown to serve as a substrate of PKB both in vitro and in vivo, and its transcriptional activity is inhibited by phosphorylation.
Catalog #A0928-02
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthesized phosphopeptide derived from human AFX around the phosphorylation site of serine 197 (A-A-SP-M-D)
SpecificityDetects endogenous levels of AFX only when phosphorylated at serine 197.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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