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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -AFX (Forkhead Box Protein O4, Fork Head Domain Transcription Factor AFX1, FOXO4, AFX1, MLLT7)

Anti -AFX (Forkhead Box Protein O4, Fork Head Domain Transcription Factor AFX1, FOXO4,
AFX1, MLLT7)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
AFX protein belongs to the forkhead protein family. It is highly homologous to the human FKHR protein, the gene of which is disrupted by the t(2;13)(q35;q14), a chromosome rearrangement characteristic of alveolar rhabdomyosarcomas. AFX contains three putative phosphorylation motif sites for protein kinase B (PKB), Thr32, Ser197, and Ser262, and it is proposed that phosphorylated AFX stays in the cytosol and is imported to the nucleus through dephosphorylation to induce target gene expression. Nuclear expression of AFX results in the suppression of various responses to hypoxia, including decreased vascular endothelial growth factor, glucose transporter 1, and erythropoietin expression. Human AFX gene is localized to chromosomal region Xq13.1.
Catalog #A0928-02B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlK562 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-EAASQDRMPQDLD, from the internal region of the protein sequence according to NP_005929.2.
SpecificityRecognizes human AFX.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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